Llandovery wing Aaron Warren has impressed in the Principality Premiership and has been brought straight into the side, while Scarlets full back Jordan Williams, centre Thomas Pascoe and front rowers Gareth Thomas and Ethan Lewis all also get their first starts of the tournament.
The Welsh youngsters are unbeaten in the Under 20 Six Nations so far with wins over Ireland and France under their belt but team manager Mark Taylor warns there is no room for complacency this weekend.
“We have made some changes to see certain combinations while keeping the backbone of the side unchanged in terms of the half backs, back row and second row," he said.
"It’s going to be another tough challenge for us on Friday. Italy are a good side, especially at home and after the experience of playing at Clermont Auvergne’s ground a fortnight ago, we are expecting another rousing atmosphere in Viterbo.
“We have made a great start to the tournament but what’s gone is gone, if it’s going to count we have to go out and put in another professional performance on Friday night. Any slacking will be exposed so we must start as we did in France and not give any ground early on if we are to do well.”
Wales Under 20 v Italy (Stadio Enrico Rocchi di Viterbo, Fri 22 Feb 6pm UK time (BBC 2 Wales)
Jordan Williams (Scarlets/ Llanelli); Aaron Warren (Llandovery) Cory Allen (Blues / Cardiff), Thomas Pascoe (Blues / Pontypridd), Ashley Evans (Ospreys/ Bridgend); Sam Davies (Ospreys / Swansea), Rhodri Williams (Scarlets/ Llandovery); Gareth Thomas (Scarlets / Carmarthen), Nicky Thomas (Ospreys / Swansea), Ethan Lewis (Blues / Cardiff), Carwyn Jones (Scarlets/ Carmarthen), Rhodri Hughes (Ospreys/ Swansea), Sion Bennett (Scarlets / Carmarthen), Daniel Thomas (Scarlets / Llanelli), Ellis Jenkins (capt, Blues / Cardiff)
Replacements: Elliot Dee (Bedwas), Nicky Smith (Ospreys / Swansea), Dan Suter (Ospreys / Bridgend), Jack Jones (Ospreys / Rovigo), James Benjamin (Bedwas), Joshua Davies (Dragons / Bedwas), Steffan Hughes (Scarlets / Llanelli), Dion Jones (Scarlets/ Llanelli)
